CHARACTERIZATION OF -AMYLASE AND PROTEASE FROM INDIGENOUS LACTOBACILLUS FERMENTUM EN17-2 AND ITS USE IN TUBER PASTE FLOUR

α-Amylase and protease are biocatalyst which can be used in paste flour to produce natural sweet paste flour. Production of these enzyme in Indonesia are limited, so, to increase those enzyme production, α-amylase and protease from indigenous bacteria are needed. This research focused in characterizat ion of α-amylase and protease from indigenous Lactobacillus fermentum EN17-2 and its use in tuber paste flour. Crude enzymes of α-amylase and protease were characterized. Tuber sources were cassava (Manihot esculenta) and sweet potato (Ipomoea batatas) with wheat (Triticum) as comparison. α-Amylase activity and reduction sugar were detected by DNS methods, while protease was detected by tyrosin method and protein degradation was detected by titration. The results show that optimum activity of αamylase from L. fermentum EN17-2 was reached at pH 5.5, 45 o C, while that optimum activity of protease was at pH 6.5, 35 o C. Stabilit y of α-amylase from L.fermentum EN17-2 was at pH 5.0-6.5, 30-60 o C; while that protease was at pH 4.5-8.0, 20-50 o C. The reduction sugar content of cassava and sweet potato paste flour were 13.50% and 23.70%, respectively, while that the reduction sugar content of those flour added α-amylase from L. fermentum EN17-2 were 32.54% and 24.30%. The protein degradation of those paste flour were 0.49% and 1.17%, respectively, while that the protein degradation of those flour added protease were 1.90 % and 1.68%. The increase of reduction sugar in those paste flour were 58.51% and 2.47%, while that protein degradation were 1.40% and 0.50%, respectively. Based on the increase of reduction sugar and protein degradation in those treated paste flour, it concluded that the characterized α-amylase and protease from L. fermentum EN17-2 was better to be used as biocatalyst in cassava pasta flour to produce natural sweet paste flour than that sweet potato.

1.  UEU-Article-15904-5_0296.pdf - 876 KB

• ISOLASI DAN PENAPISAN BAKTERI SELULOLITIK DARI BERBAGAI JENIS TANAH SEBAGAI PENGHASIL ENZIM SELULASE
• UJI AKTIVITAS PENGHAMBATAN ENZIM TIROSINASE DARI FRAKSI ETIL ASETAT DAUN SUKUN (Artocarpus altilis [Park.] Fosberg.)
• SCREENING ANGIOTENSIN CONVERTING ENZYME (ACE) INHIBITOR ACTIVITY OF ANTIHYPERTENSIVE MEDICINAL PLANTS FROM INDONESIA
• ISOLASI DAN PENAPISAN BAKTERI SELULOLITIK DARI BERBAGAI JENIS TANAH SEBAGAI PENGHASIL ENZIM SELULASE
• CANDIDA ANTARCTICA LIPASE B SYNTHETIC GENE : A BIOINFORMATICS ANALYSIS
• PROFIL RINGKASAN LITABMAS : EVALUASI AKTIVITAS ANTIMIKROBA DAN KEMAMPUAN PROBIOTIK SERTA UJI ENZIMATIS DARI YEAST RHODOTORULA MUCILAGINOSA GALUR PK-S20
• IN-VITRO Α-GLUCOSIDASE INHIBITORY ACTIVITY OF LITSEA PETIOLATA HK. F
• PROFIL RINGKASAN LITABMAS: DETEKSI DAN KLONING GEN PENYANDI FITASE DARI YEAST RHODOTORULA MUCILAGINOSA GALUR PK-S20 DALAM EFESIENSI PERBAIKAN KUALITAS PAKAN
• UJI INHIBISI ENZIM -GLUKOSIDASE DAN -AMILASE DARI EKSTRAK ETANOL 70% BIJI JAGUNG UNGU (ZEA MAYS VAR. CERATINA KULESH)
• OPTIMASI DAN IDENTIFIKASI YEAST ISOLAT BL-20 DAN PT-20 SEBAGAI KANDIDAT PENGHASIL ENZIM PROTEASE

• PENGARUH PERESEPAN ELEKTRONIK TERHADAP MUTU LAYANAN FARMASI DI RUMAH SAKIT �X� JAKARTA BARAT
• LAPORAN MAGANG; PENGELOLAAN PERBEKALAN FARMASI DI INSTALASI FARMASI RUMAH SAKIT PRATIWI (IBU & ANAK) KOTA TANGERANG TAHUN 2013
• GAMBARAN PENCATATAN DAN PELAPORAN PERBEKALAN FARMASI DI RUMAH SAKIT SIAGA RAYA
• ANALISIS PENGARUH PROFITABILITAS, UKURAN PERUSAHAAN, LEVERAGE DAN NILAI PERUSAHAAN TERHADAP PRAKTIK PERATAAN LABA (STUDI EMPIRIS : PADA PERUSAHAAN INDUSTRI FARMASI YANG TERDAFTAR DI BURSA EFEK INDONESIA PERIODE TAHUN 2008-2011)
• ANALISIS PENGARUH LEVERAGE, STRUKTUR MODAL DAN PROFITABILITAS TERHADAP PRICE EARNING RATIO (PER) PADA PERUSAHAAN FARMASI YANG TERDAFTAR DI BURSA EFEK INDONESIA PERIODE 2002-2008
• ANALISIS FAKTOR � FAKTOR YANG MEMPENGARUHI STRUKTUR MODAL PADA PERUSAHAAN FARMASI DI BURSA EFEK INDONESIA (PERIODE 2006 � 2009)
• HUBUNGAN ANTARA MOTIVASI KERJA DAN KINERJA PETUGAS PELAYANAN RESEP OBAT JADI DI INSTALASI FARMASI RSUD CENGKARENG
• ANALISA PENGARUH PROFITABILITAS, TOTAL ASSET, INVENTORY TURNOVER, FINANCIAL LEVERAGE DAN OPINI AUDITOR TERHADAP PRAKTIK PERATAAN LABA PADA PERUSAHAAN FARMASI YANG TERDAFTAR DI BE1 PERIODE TAHUN 2007 - 2010
• GAMBARAN UMUM SISTEM MANAJEMEN LOGISTIK FARMASI DI RUMAH SAKIT ASSHOBIRIN TANGERANG TAHUN 2013
• PENATALAKSANAAN PENYIMPANAN FARMASI DI INSTALASI FARMASI RUMAH SAKIT MEDIKA PERMATA HIJAU JAKARTA BARAT